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Re-using a needle for both bac water draw and peptide vial entry can carry microbial load from the peptide vial back into the bac water bottle

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Considering the position of the Gly 1228, we could argue that the mutation G1228D will trigger a steric hindrance with T998 that is tightly constrained by F583 and cannot move easily in the apo and LTC 4 -bound conformations

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