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Importantly, phosphorylation at Ser1025 did not alter global microtubule stability, as indicated by unchanged acetylated-tubulin levels, but specifically enhanced microtubule anchoring at the Golgi and increased EB1 signals at the cell periphery (Figure 4B, C)

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Rao Q, Zhang T, Dai M, Li B, Pu Q, Zhao M, et al

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After blocking with bovine serum albumin (BSA), the membranes were detected using anti-Nrf2 (1:5,000, Abcam), anti-GPX4 (1:1,000, Abcam), anti-SLC7A11 (1:1,000, Abcam), anti-DHODH (1:1,000, Abcam) and anti--actin (1:10,000, Abclone)