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When working with an eukaryotic expression system that may contain unknown agents and factors interfering the binding of target protein, it is recommended to dilute the culture supernatant 1:1 or 1:2 with the Equilibration buffer (for example, 20 mM sodium phosphate, 0.5 M NaCl, pH 7.4)

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The applicator is positioned over the treatment area, and a light suction gently pulls the area into the applicator as it freezes the fat cells

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