Gritstone Biotech employed Venezuelan equine encephalitis virus-based self-amplifying mRNA vector in a lipid nanoparticle called GRT-R910 encoding both the prefusion stabilized full-length SARS-CoV-2 spike and additional conserved T-cell epitopes from ORF3a, NP and membrane (Table 3)
I'll have to try again tomorrow but if you could send a link, it would be very much appreciated
Testicular protein extracts were prepared as follows: Testicular tissue (500 mg) was homogenized in ice-cold protein lysis buffer (pH 7.5) containing 50 mM Tris, 250 mM sucrose, 10 mM ATP, 5 mM MgCl 2 , 1 mM DTT, and protease inhibitors (10 g.ml 1 of antipain, aprotinin, leupeptin and pepstatin A, as well as 20 M PMSF)
Incretin Analogs for Weight Management in Adults Without Diabetes
Something else to consider
These vegetables contain sulforaphane and indole-3-carbinol compounds that specifically upregulate Phase 2 liver detoxification enzymes