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Mistake 3: improper storage Leaving reconstituted peptide at room temperature, even briefly, accelerates degradation

10.1016/j.abb.2021.108918 Arch

EN: Visualization, Writingreview and editing

Funding This work was supported by INOVA FIOCRUZ (VPPCB-005-FIO-20-2-115), Coordenao de Aperfeioamento de Pessoal de Nvel SuperiorCAPES (88881.504749/2020-01

After FOXO4-DRI treatment, the staining of FOXO4 and p53-pS15 became weaker in the nucleus at 48 h and then disappeared with an accumulation in the cytoplasm at 72 h, especially in keloid fibroblasts