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NNMT consumes both nicotinamide (an NAD+ precursor) and SAM (the universal methyl donor) to produce MNA

It is not approved or intended for human or animal use, and any discussion of biological activity refers only to preclinical or in vitro research

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Nicotinamide N-methyltransferase as a potential therapeutic target in neurodegenerative diseases

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After washing with cell lysis buffer, bound protein was eluted with 20 mM HEPES pH 7.0, 500 mM NaCl, and 250 mM imidazole