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Gradient elution of analytes was carried out with 0.1% formic acid in water (C) and 0.1% formic acid in acetonitrile (D), or 5 mM ammonium formate in water (A) and acetonitrile (B) at a flow rate of 0.25 ml/min

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Research Applications Appetite and feeding behavior: The established primary application characterizing NPY/AgRP-mediated orexigenic signaling in preclinical models using the compound most reliably activating those circuits Energy homeostasis: Ghrelins role in energy balance, adipogenesis, and metabolic adaptation during caloric restriction

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