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Whole-cell patch-clamp recordings were performed in oxygenated standard ACSF that contained (in mM) 129 NaCl, 1.2 KH 2 PO 4 , 3 KCl, 2.4 CaCl 2 , 3 HEPES, 1.3 MgSO 4 , 10 glucose, and 20 NaHCO 3 (PH 7.37.4, 300310 mOsm, oxygenated with 95% O 2 and 5% CO 2 ) at 25 C

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Development of a direct contact astrocyte-human cerebral microvessel endothelial cells blood-brain barrier coculture model