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Discussion Chronic diabetic wounds represent a huge healthcare challenge, necessitating sustained and synchronized regulation of the wound microenvironment simultaneously with tissue reconstruction processes

Results clearly present the chemical potentiation of LASSS on physiological functions of HGF, as revealed by enhanced receptor-binding affinity and nitration-dysfunction resistance through interaction/reaction with LASSS under physiological conditions

To evaluate the healing efficacy, a comprehensive experiment was conducted on diabetic wounds treated with various agents, including: i) PBS as negative control (NC group), ii) ROS-responsive hydrogel matrix (G group), iii) monomer copper peptides M-CuP solution (M-CuP group), iv) dimeric copper peptides D-CuP solution (D-CuP group), v) ROS-responsive hydrogel with monomer copper peptides M-CuP (G/M-CuP group), and vi) ROS-responsive hydrogel with dimeric copper peptides (G/D-CuP group)

Using a leave-one-out cross-validation (LOOCV) framework, we first applied logistic regression to rank metabolites based on their absolute model weights, identifying those most predictive of symptom improvement

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The peptide reaches the epidermis and superficial dermis but struggles to reach follicle depth