A 12.5 g cell pellet was resuspended in 40 ml buffer B (25 mM Tris-HCl, pH 7.5, 10% glycerol, 0.01% Igepal CA-630, 2 mM 2-Mercaptoethanol, 5 mM Imidazole) containing 500 mM KCl and a cocktail of protease inhibitors (PIs) (2 g/ml each of aprotinin, chymostatin, leupeptin, and pepstatin A) and lysed by sonication (QSonica Q700) at an amplitude of 40 for 2.5 min with 20 s pulses
2006 doi: 10.1016/j.joca.2005.08.009
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High-Dose Protocols: Some dermatologists may administer doses up to 2400 mg per session, especially for individuals with stubborn pigmentation or darker skin tones
Biochemical Journal, 301(3), 769-775
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