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coli K-12 strain was selected as a Gram-negative control due to its well-characterized lipopolysaccharide (LPS)-induced inflammatory properties and regulatory role in EMs pathogenesis [27, 44]

Findings from empirical studies show that lactate concentration in the TME reaches 3040 mM, exerting dual immunosuppressive mechanisms through microenvironmental acidification and direct molecular signaling (15)

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doi: 10.1038/npp.2017.150, 113 PhelanS