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Finally, we investigated whether a decreased leakage of mitochondrial ROS was due to direct antioxidant properties of tested therapeutics, or whether these compounds influence the ROS leakage by modulation of activity and efficiency of mitochondrial respiratory chain.25 We measured mitochondrial respiration of the cells maintained in the complete medium in a basal state and during maximal stimulation with an uncoupling agent FCCP, and basal proton leakage during inhibition of Complex V with oligomycin

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