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Cells treated with test compounds were washed with PBS and incubated with 10 M 2,7-dichlorofluorescein diacetate (DCFH-DA) at 37 C for 30 min, followed by washing with PBS, digestion with trypsin, and collection

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Above the high-risk line (300 ug/mL at 4 hours) indicates a high risk of liver failure (massive ingestion), which may warrant even more aggressive therapy (discussed below)