HPLC measurement of DA, DOPAC and HVA DA and metabolites were separated by injecting 50 l of sample into a Microsorb C18 column (100 4.6 mm column, Analytical Column, Waters) and a mobile phase containing 130 mM NaH 2 PO 4 , 2 mM NaCl, 0.1 mM EDTA, 2 mM OSA (1-octanesulphonic acid sodium) and 12.5% (v/v) methanol, pH 3.71, at a flow rate of 1 ml min 1
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Direct injection of DA (up to 1 mol) into the rat striatum led to the loss of dopaminergic nerve terminals and the formation of cysteinyl adducts of DA in a DA-dose-dependent manner, and this was alleviated by co-administration of glutathione (GSH) or ascorbate [36,37,38]
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Milk thistle
It also inhibits the production of melanin