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After a final wash with PBS, we mounted the coverslips on microscope slides used for imaging on a Zeiss LSM 870 confocal microscope, as we reported recently 45

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Tombes, R., Brokaw, C

Test subjects were included in the study if they were at least 18 years of age with a type 2 diabetes diagnosis and stable treatment with metformin, thiazolidinediones, and/or sulfonylureas, for at least 90 days prior to the initiation of the experiment