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The peptide itself has a high affinity for divalent copper (Cu), and in physiological conditions it spontaneously forms the GHK-Cu complex

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The pellet was resuspended in 2 ml ammonium chloride/Tris-chloride (pH 7.2) (erythrocyte lysing buffer), incubated at room temperature for 5 min, then supplemented with 1 ml FCS and centrifuged subsequently at 1433 r.p.m

Wang ZJ, Han WN, Chai SF, Li Y, Fu CJ, Wang CF, Cai HY, Li XY, Wang X, Hlscher C, Wu MN (2024) Semaglutide promotes the transition of microglia from M1 to M2 type to reduce brain inflammation in APP/PS1/tau mice