Take a new syringe from the package (syringes are single use only, call or visit us to get refills on syringes when needed) Draw up 0.25ml of the red Vitamin B12 solution (measure from the top of the black plunger). Pull up the skin between the shoulder blades to create a skin tent Insert the whole needle into the skin (pointing downward towards the body), make sure the needle has not exited the skin on the other size of the skin tent
doi: 10.1007/s11695-018-3493-4
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So a reconstituted vial is not germ-proof
BAC water includes 0.9% benzyl alcohol as a bacteriostatic preservative, while sterile water has no preservative and saline has sodium chloride instead
However, serum B12 is a crude measure and can be normal even in the presence of functional deficiency