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Briefly, a 1 ml reaction mix (500 nM TGR, 100 M NADPH, and 2050 M inhibitor) was incubated at room temperature for 30 min for fast inhibitors, or 2 h for slow inhibitors

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JAMA Psychiatry 72:757766 de Wit M, Jones DG, Sessler CN, Zilberberg MD, Weaver MF (2010) Alcohol-use disorders in the critically ill patient