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Initially (untreated cells for initial time point) or after 4 days (with varying treatment conditions), 40 L of Cell Titer Glo reagent (Promega) was added to each well, mixed briefly, and the luminescence read on a luminometer (Molecular Devices)

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Asparagine followed by larger amino acids deamidates more slowly because the larger side chains sterically hinder the cyclization reaction that drives deamidation

Dark spots on areas like my elbows and knees have also improved

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Less snacking and overeating: Directly influences brain regions tied to cravings and hunger