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Particle auto-picking was performed using a pretrained model in the crYOLO software package, followed by particle extraction in a box-size equivalent to 320 and Fourier down-sampled to 64 pixels

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They are trying to understand whether these peptides overlap, whether they complement one another, and whether using both makes more sense than choosing only one

They're not here yet, but the trajectory is clear

However, combining the two spectral libraries provides the best coverage with 30,912 phosphopeptides and 14,835 sites (Supplementary Data 3)

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