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Evaluation of antioxidant capacity of SAC DPPH Free Radical Scavenging Capacity Assay Kit (Nanjing Jiancheng Bioengineering Institute, A153-1-1, Nanjing, China): Prepare the working solution according to the kit instructions, mix with different concentrations of SAC solution, incubate in the dark at 25 for 30 min, centrifuge at 4000 rpm for 5 min, transfer 100L to a cuvette, zero with 80% methanol, and measure absorbance at 517 nm

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The cervix, or lower portion of the uterus, remains in place

doi: 10.1016/j.vaccine.2016.03.079 52 SiddiquiAAPhillipsTCharestHPodestaRBQuinlinMLPinkstonJRet al

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It takes approximately 3 hours to perform the procedure